Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Clinical characteristics and related data from included studies in this systematic review

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques:

Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Features of podoplanin immunoexpression in different odontogenic lesions

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques: Expressing

Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Forest plot comparing the PDPN immunoexpression of A OKC vs DC. B Funnel plot to check the publication bias. OKC=Odontogenic keratocyst, DC= Dentigerous cyst

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques:

Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Podoplanin immunoexpression in odontogenic lesions. PDPN is strongly expressed in DF, OKC, COC, AMs, AMU, AMPe, AMC, AOT, CEOT, AF and AFO. Its expression has been shown to be negative in GOC and OM. Furthermore, PDPN has been shown to participate in cell adhesion, migration and invasion through association with ERM proteins, which are expressed in different OL such as OKC, OOC, COC, AMs, AOT, CEOT, ODS and AF. IHC: Immunohistochemistry; OL: Odontogenic lesions; PDPN: Podoplanin; ERM: Ezrin, Radixin, Moesin; EZR: Ezrin; RDX: Radixin; MSN: Moesin; RhoA: Ras homolog gene family, member A; DF: Dental follicle; OKC: Odontogenic keratocyst; OOC: Orthokeratinized odontogenic cyst; COC: Calcifying odontogenic cyst; GOC: Glandular odontogenic cyst; AMs: Ameloblastoma solid; AMU: Ameloblastoma, Unicystic; AMPe: Ameloblastoma, Peripheral; AMC: Ameloblastic Carcinoma; AOT: Adenomatoid odontogenic tumor; CEOT: Calcifying epithelial odontogenic tumor; AF: Ameloblastic fibroma; AFO: Ameloblastic fibro-odontoma; ODS: Odontoma; OM: Odontogenic myxoma [ – , , ]

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques: Expressing, Migration, Immunohistochemistry

Journal: International journal of cancer

Article Title: Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma.

doi: 10.1002/ijc.29464

Figure Lengend Snippet: Figure 2. CAFs expressing PDPN invade further in the collagen matrix and promote cancer cell invasion. (a, b) Examples (a) and quantifica- tion (b) of the invasion of CAFs overexpressing WT-PDPN cultured alone for 72 hr using the collagen invasion assay model. (c–e) Examples (c) and quantification (d, e) of the invasion of cocultures of A549 cancer cells and CAFs overexpressing WT-PDPN for 72 h using the colla- gen invasion assay model. (f, g) Examples (f) and quantification (g) of the invasion of CAFs with the knockdown of PDPN that were cultured alone for 48 hr using the collagen invasion assay model. (h–j) Examples (h) and quantification (i, j) of the invasion of cocultures of A549 cancer cells and CAFs with the knockdown of PDPN for 48 hr using the collagen invasion assay model. The error bars indicate 6 SD. The scale bars represent 50 mm. *p < 0.05, ***p < 0.001. [Color figure can be viewed in the online issue, which is available at wileyonlineli- brary.com.]

Article Snippet: PDPN staining of the human surgical specimens was performed using anti-human mouse monoclonal PDPN antibody (D2-40; Acris Antibodies).

Techniques: Expressing, Cell Culture, Invasion Assay, Knockdown

Journal: International journal of cancer

Article Title: Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma.

doi: 10.1002/ijc.29464

Figure Lengend Snippet: Figure 3. Rho-ROCK function is required for the invasion of CAFs expressing PDPN in the collagen matrix. (a) Quantification of the invasion of CAFs overexpressing PDPN-del.IC or WT-PDPN cultured alone for 72 hr using the collagen invasion assay model. (b–d) Examples (b) and quantification (c, d) of the invasion of cocultures of A549 cancer cells and CAFs overexpressing PDPN-del.IC or WT-PDPN for 72 hr using the collagen invasion assay model. (e) Relative activity of RhoA using the G-LISA assay in CAFs overexpressing PDPN-del.IC or WT-PDPN. (f) Western blot analysis showing the phosphorylation of ERM, myosin light chain and cofilin in CAFs overexpressing PDPN-del.IC or WT-PDPN. The expression of a-tubulin was used as a control for protein loading. (g) Quantification of the invasion of A549 cancer cells and CAFs over- expressing WT-PDPN cultured alone with the ROCK inhibitor Y27632 for 72 hr using the collagen invasion assay model. (h, i) Quantification of the invasion of co-cultures of A549 cancer cells and CAFs overexpressing WT-PDPN with Y27632 treatment for 72 hr using the collagen invasion assay model. The error bars indicate 6 SD. The scale bars represent 50 mm. *p < 0.05, **p < 0.01, ***p < 0.001. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: PDPN staining of the human surgical specimens was performed using anti-human mouse monoclonal PDPN antibody (D2-40; Acris Antibodies).

Techniques: Expressing, Cell Culture, Invasion Assay, Activity Assay, Western Blot, Phospho-proteomics, Control

Journal: International journal of cancer

Article Title: Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma.

doi: 10.1002/ijc.29464

Figure Lengend Snippet: Figure 4. CAFs expressing WT-PDPN invade the mouse lung parenchyma and promote cancer cell invasion. (a) Examples of non-invaded (upper) and invaded (lower) CAFs (left) and A549 cancer cells (right) in the mouse lung parenchyma at 24 hr after injection with immuno- histochemical staining for Venus-CAFs or A549 cancer cells (red) and CD31 (brown). (b) Examples of noninvaded (left) and invaded (right) CAFs in the lung parenchyma at 24 hr after injection with immunofluorescence staining for Venus-CAFs (red) and CD31 (green). (c) Examples of invaded CAF and A549 cancer cells in the lung parenchyma at 24 hr after injection with immunofluorescence staining for Venus-CAF (red), mRFP-A549 cells (blue) and CD31 (green). (d) Quantification of CAFs overexpressing WT-PDPN injected with A549 cancer cells just after injection (n 5 5, each). (e) Quantification of A549 cancer cells injected alone and with control CAFs or CAFs overexpressing WT-PDPN just after injection (n 5 5, each). (f) Quantification of invaded CAFs overexpressing WT-PDPN injected alone 24 hr after injection (n 5 5, each). (g) Quantification of invaded control CAFs and CAFs overexpressing WT-PDPN injected with A549 cancer cells 24 hr after injection (n 5 5 and 6, respectively). (h) Quantification of invaded A549 cancer cells injected with control CAFs or CAFs overexpressing WT-PDPN and injected alone 24 hr after injection (n 5 5, 6 and 5, respectively). The error bars indicate 6 SEM. The scale bars represent 20 mm. *p < 0.05. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: PDPN staining of the human surgical specimens was performed using anti-human mouse monoclonal PDPN antibody (D2-40; Acris Antibodies).

Techniques: Expressing, Injection, Staining, Control

Journal: International journal of cancer

Article Title: Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma.

doi: 10.1002/ijc.29464

Figure Lengend Snippet: Figure 5. PDPN-positive CAFs in clinical samples of lung adenocarcinoma. (a) Scheme showing lung adenocarcinoma invasion into the vis- ceral pleura in clinical samples. While the lung tumor is growing up, it invades the outside of the lung, which is called the pleural invasion. In the central area of the tumor, PDPN-expressing CAFs were observed. At the invasive front of the tumor, PDPN-expressing CAFs were also found. (b) Examples of PDPN expression (brown) in CAFs and the elastic layer in the visceral pleura (green) at the invasive front (upper left; scheme, upper right) and in the central area (lower left) using immunohistochemical staining. (c) Correlation between PDPN-expressing CAFs and pleural invasion at the invasive front. (PDPN(1)CAFs/pleural invasion: 2/2, n 5 47; 2/1, n 5 9; 1/2, n 5 3; and 1/1, n 5 19). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: PDPN staining of the human surgical specimens was performed using anti-human mouse monoclonal PDPN antibody (D2-40; Acris Antibodies).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Cancer Research

Article Title: Podoplanin-Positive Fibroblasts Enhance Lung Adenocarcinoma Tumor Formation: Podoplanin in Fibroblast Functions for Tumor Progression

doi: 10.1158/0008-5472.can-10-3228

Figure Lengend Snippet: Figure 3. Podoplanin is a functional protein in the fibroblast- induced augmentation of tumor formation. A, Western blot analysis showing PDPN expression in control or podoplanin shRNA-infected hVAFs. B, mRNA levels of podoplanin in control or podoplanin shRNA-infected hVAFs. C, colony formation assay. Colonies with a diameter greater than 200 mm were counted for A549 cells with either control or podoplanin shRNA-infected hVAFs. Error bars show the mean SD. Data shown are representative of multiple repeats. Asterisk, P < 0.01 using the Student's t test. D, tumor formation rate measurement after the s.c. injection of 1 104 A549 cells with control or podoplanin shRNA-infected hVAFs into SCID mice (n ¼ 8–10). Asterisk, P < 0.01; double asterisk, P < 0.01 using the Student's t test. E, flow cytometric analysis of control or wild-type podoplanin (WT-PDPN)–infected hVAFs. Thin line, IgG control; dotted line, control hVAFs; bold line, WT- PDPN overexpressed hVAFs. F, tumor formation rates after the s.c. injection of 5 104 A549 cells with control or WT-PDPN overexpressed hVAFs into SCID mice (n ¼ 4).

Article Snippet: The blots were incubated overnight at 4 C with anti-human mouse monoclonal podoplanin antibody (D2-40, Signet) or polyclonal goat actin antibody (Santa Cruz).

Techniques: Functional Assay, Western Blot, Expressing, Control, shRNA, Infection, Colony Assay, Injection

Journal: Cancer Research

Article Title: Podoplanin-Positive Fibroblasts Enhance Lung Adenocarcinoma Tumor Formation: Podoplanin in Fibroblast Functions for Tumor Progression

doi: 10.1158/0008-5472.can-10-3228

Figure Lengend Snippet: Figure 4. Podoplanin-positive stromal fibroblasts in human adenocarcinoma as a prognostic factor. A, immunostaining of podoplanin in stromal fibroblasts of human lung adenocarcinoma patients with tumor size of 3 cm or less. B, number of patients and rates of clinicopathologic factors in relation to podoplanin expression in the stromal fibroblasts evaluated using the c2 test. Percentage shows rates of each factor within podoplanin expression. D, disease-free interval curve (C) and overall survival curve (D) of podoplanin expression in stromal fibroblasts of human lung adenocarcinoma patients with a tumor size of 3 cm or less.

Article Snippet: The blots were incubated overnight at 4 C with anti-human mouse monoclonal podoplanin antibody (D2-40, Signet) or polyclonal goat actin antibody (Santa Cruz).

Techniques: Immunostaining, Expressing

Journal: Scientific reports

Article Title: Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels.

doi: 10.1038/s41598-022-26041-0

Figure Lengend Snippet: Figure 2. Effects of cell ratio and vascular endothelial growth factor-C (VEGF-C) concentration on the mesh pattern by lymphatic endothelial cells (LECs) co-cultured with adipose-derived stem cells (ASCs). (a–l) Representative images of LECs co-cultured with ASCs and immunostained for podoplanin (a glycoprotein expressed by LECs). The LECs were cultured in medium containing 0 ng/mL VEGF-C (a–d), 25 ng/mL VEGF-C (e–h) or 50 ng/mL VEGF-C (i–l), and the ratio of LECs to ASCs was either 1:4 (a,b,e,f,i,j) or 3:2 (c,d,g,h,k,l). High-power images of (a,c,e,g,i,k) which were skeletonized by ImageJ software to evaluate branches and branch lengths are shown in (b,d,f,h,j,l). LECs co-cultured with ASCs at a ratio of 1:4 in 0 ng/mL VEGF-C or 25 ng/mL VEGF-C generated elongated structures but did not form ‘islands’ (a,b,e,f). Similar results were obtained when LECs were co-cultured with ASCs at a ratio of 1:4 in 50 ng/mL VEGF-C, although the LEC mesh pattern was denser than that obtained in the lower concentrations of VEGF-C (i,j). LECs co-cultured with ASCs at a ratio of 3:2 in 0 ng/mL VEGF-C formed island-like structures with a dense LEC mesh pattern (c,d). LECs co-cultured with ASCs at a ratio of 3:2 in 25 ng/mL VEGF-C or 50 ng/mL VEGF-C formed more prominent island-like structures containing a dense and fine LEC mesh pattern (g,h,k,l). (m) Density of branches in the LEC mesh pattern (number of branches per mm2). Significantly more branches were observed for a LEC/ASC ratio of 3:2 than for a ratio of 1:4 (P < 0.0001, two-way analysis of variance [ANOVA]). However, the concentration of VEGF-C had no significant effect on junction formation (P = 0.83, two-way ANOVA). Pairwise comparisons showed that branch density in the 3:2 ratio/25 ng/mL VEGF-C group was significantly higher than that in the 1:4 ratio/0 ng/mL VEGF-C group or the 1:4 ratio/25 ng/mL VEGF-C group (P < 0.05). All conditions were quantified and compared. Non-significant differences were not denoted on the graphs. The data are presented as the mean ± standard deviation (n = 8 per group). * P < 0.05. (n) LEC mesh pattern branch length (mm/mm2). Branch length was longer for a LEC/ASC ratio of 3:2 than for a ratio of 1:4 (P = 0.002, two- way ANOVA). However, VEGF-C had no significant effect on branch length (P = 0.76, two-way ANOVA). All conditions were quantified and compared. Non-significant differences were not denoted on the graphs. The data are presented as the mean ± standard deviation (n = 8 per group).

Article Snippet: Then, the slides were incubated with anti-human podoplanin mouse monoclonal primary antibody (clone D2-40, #413451, Nichirei Biosciences, Japan; 1:10) overnight at 4 °C.

Techniques: Concentration Assay, Cell Culture, Derivative Assay, Software, Generated, Standard Deviation

Journal: Scientific reports

Article Title: Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels.

doi: 10.1038/s41598-022-26041-0

Figure Lengend Snippet: Figure 3. Lymphatic vessel-like structures were evident in a three-layered LEC/ASC sheet (1:4 ratio) 2 weeks after transplantation onto rat gluteal muscle. (a) Temperature-responsive culture dish coated with Poly(N- isopropylacrylamide) (PIPAAm), temperature-responsive polymer used to construct cell sheet. At temperature of 37 °C, cells adhere to surface of dish, and temperature decreased to 20 °C enable cells detach from dish preserving their cell–cell junctions, cell surface proteins and extracellular matrix without need of enzymatic treatment which breaks cell–cell junctions. (b) Observation of three cell sheets stacked before implantation by stereo microscope. (c) Three LEC/ASC sheets were stacked and transplanted onto rat gluteal muscle in vivo, and immunostaining for podoplanin was performed 2 weeks later. The low-power image shows LECs positive for human podoplanin. CS cell sheet. (d) High-power image showing a few lymphatic vessel-like structures composed of LECs. (e) Positive control image of human podoplanin in human small intestine.

Article Snippet: Then, the slides were incubated with anti-human podoplanin mouse monoclonal primary antibody (clone D2-40, #413451, Nichirei Biosciences, Japan; 1:10) overnight at 4 °C.

Techniques: Transplantation Assay, Polymer, Construct, Preserving, Microscopy, In Vivo, Immunostaining, Positive Control